1. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. edu) to be added as a trained user in iLab. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 0 (IBM Corporation, Armonk, NY, USA). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 00 USD. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). Settings not required by a reader are indicated by ‘---’. The integration time suggested is 5-10 sec. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). no. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Step 2. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. SpectraMax M5e Multi-Mode Microplate Reader. Note: In this user guide, all references to SpectraMax Multi-Mode. Lower limits of detection for ATP determined using the ATPlite . The following document is intended to demonstrate setup of this instrument. 0200-0729. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. VWR offers biophotometers for your lab's needs. . Unit comes with software and computer. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. spr SpectraMax iD3 SpectraTest ABS2 v1. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. With optimized reagents, option to operate the. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. 72 MB. The SpectraMax M2 and M2e are versatile and reliable. EarlyTox Live Cell Assay (6. NORTH AMERICA. . Microplate Reader Shelf. . SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. Spectranet customer portal. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. These all-inclusive assay kits are ready to use in assay validation or screening. Softmax Pro GxP Software 6. . The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 40266 Email: drugdiscoverytech@lifetech. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. . +44-118-944-8000. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax M5 is a microplate reader that can be used for various applications,. Features. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 4. Detection of pro-inflammatory factors. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 304 signal level saturation. Microplate Readers include the M3, M4,. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. . l. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. When the user applies a set temperature of 37°C, the top is heated to 37. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. , contact Molecular. SpectraMax L Microplate Reader provides medium to high throughput. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Read fluorescence polarization at room temperature (RT, 19–25°C). The Spectr. 3. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 313 reference gain check fail. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. 95. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. Set the excitation at 531 nm and emission at 595 nm. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 1. . User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Multimode Reader Comparison Chart. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Third this recommendation. 0 out of 5 $ 15,999. ATP standard curves read on SpectraMax microplate readers. 7 nM EGF, the EC 80 concentration determined from. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Examples: “Press carefully to engage,” “ Do not press down. In addition, the SpectraMaxMolecular Devices. Table of Contents. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. HTRF-compatible SpectraMax Multi-Mode Readers. Select the fluorescence option and read the total fluorescence. . While they play an important role in generating data from a wide range of. Cytokines in medium released. spr SpectraMax iD5 SpectraTest ABS2 v1. Find regional support. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . Cables. 3: The cuvette chamber. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. . For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. Example: [Drawer]. 0 mm wide, and heights from 14 mm to 15 mm. We would like to show you a description here but the site won’t allow us. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Our absorbance plate readers feature our PathCheck Sensor technology and. 2. I have been using the SpectraMax M5 since 2011. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. The SoftMax® Touch Software is installed in. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). $4,499. The plate reader can measure multiple readouts, including UV. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Five preferred modes for diverse bioresearch applications. Table of Contents. 2. . These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. ScanLater Western Blot Detection Cartridge. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. Maximum signal with lowest background. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. com A. Wavelength optimization. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Our absorbance plate readers feature our PathCheck Sensor. D 1 1. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Customer Satisfaction is our number one goal All lab equipment in stock. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMax M5, Analyst HT or Analyst GT. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 1. ® reader to the computer. Softmax Pro GxP Software 6. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. 2. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Wavelength Range. 5. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. 3. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. edu) to be added as a trained user in iLab. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M5 and the Analyst GT. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Description 1. 2. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Recertification is recommended every two years. acquired on a SpectraMax M5 (Molecular Devices). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. UV/Vis Photometric Performance:. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. With optimized reagents, option to operate the readers. It is available to all researchers. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. . LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. This application note describes how to. spr SpectraMax Plus 384. 190-1000 nm, tunable in 1 nm increments. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. For very concentrated samples, use a minimum2. Sensitive fluorescent quantitation of DNA with the Quant-iT. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 1. The corrected absorbance is within 5% of the. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . 0 out of 5 $ 5,999. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. . SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. Last lab had ours for years and never had a problem with it. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 00 USD. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Sale. . Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. free tracer decreases, resulting in an. Wavelength optimization. 00 USD. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. D 47 7. 1. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. 5. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. 19. 1. The dynamic range of the assay was approximately 5 orders of magnitude. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Examples: “Press carefully to engage,” “ Do not. 72 MB. Bookings are. you can use a resource. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Open the Softmax Pro software and select the FP option. Setup recommendations for SpectraMax® M5e. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Add to Cart. SpectraMax M5 microplate reader. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Optimized for fluorescence microplate readers. S. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Add to Cart. 6. All reagents were brought to room temperature before use. . 9000-0756. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. 7. Find regional support. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Here, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. SoftMax Pro. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. . Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 190-1000 nm, tunable in 1 nm increments. The KeySpan USA-19HS is often required with new Windows 7. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Add to Cart. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax ABS Plus SpectraTest ABS2 v1. Introduction. In the M2, M5 and Plus. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. . spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. e. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. . Step 4. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Monday to Friday 8am - 5pm GMT. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. com A. Open the SoftwarePro® Data Acquisition & Analysis software,. 7. The M5 can read volumes down to 2uL with the uMax Low. Small, unbound molecules rotate rapidly in solution (top), while large,. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). 4. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Also refer to this Multimode Reader Comparison Chart. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). D 1 1. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 3: The cuvette chamber. D 1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. ORDER. 4. Lift up the lid over the chamber to insert or remove a cuvette. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Detection cartridge modularity allows for easy addition of new read modes within minutes without. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 4. Cavicide (Warehouse, Cat # 79300360) 6. Call 516-939-0888 to check current. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. The . As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE.